Application

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

Wstern Blot: 1µg/mL (Hsu, 1997a; Hsu, 1997b)

Flow Cytometry

Immunofluorescence Immunoprecipitation: native protein (2ug/mg protein lysate).

Immunohistochemistry (frozen and paraffin): 2-4ug/mL for 30 minutes at room temperature.

Optimal working dilutions must be determined by end user.

This Anti-Bcl-X Antibody, clone 2H12 is validated for use in FC, IF, IP, WB, IH for the detection of Bcl-X.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bcl-X has two isoforms, Bcl-XL (long), a 241-aa protein, and Bcl-XS (short), a 178-aa protein lacking a 63 aa domain that is well conserved among members of the Bcl-2 family.

Immunogen

Synthetic peptide, aa 3-14 of human Bcl-X protein. This amino acid sequence is shared by human and murine Bcl-X protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified immunoglobulin in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Format: Purified

Specificity

MAB4625 recognizes a protein of 27 kDa, identified as the Bcl-X protein. Reacts with both Bcl-XS and Bcl-XL.

Storage and Stability

Maintain at 2-8°C for up to 12 months.